GRAY WATER TO GREEN GOLD: CHARACTERIZATION AND POTENTIAL OF POLYHYDROXYALKANOATE-PRODUCING MICROBES FROM INDUSTRIAL EFFLUENTS

R AFRIDI; F SHIREEN; S KHAN; S SARDAR; SM SHAH; A BATOOL; Q ALI; M ASHFAQ; A AHMAD; A ULLAH

doi:10.64013/bbasr.v2025i1.105

Authors

R AFRIDI Sarhad Institute of Allied Health Sciences (SUIT) Peshawar, 25000 Peshawar, Pakistan
F SHIREEN Sarhad Institute of Allied Health Sciences (SUIT) Peshawar, 25000 Peshawar, Pakistan/Department of Allied Health Sciences, Iqra National University (INU) Peshawar, 25000 Peshawar, Pakistan
S KHAN School of Resources and Environmental Engineering, East China University of Science and Technology ,Shanghai, 200237, China
S SARDAR Sarhad Institute of Allied Health Sciences (SUIT) Peshawar, 25000 Peshawar, Pakistan
SM SHAH Department of Health and Biological Sciences, Abasyn University Peshawar, 25000 Peshawar, Pakistan
A BATOOL Department of Plant Breeding and Genetics, Faculty of Agricultural Sciences, University of the Punjab, P.O BOX. 54590, Lahore, Pakistan
Q ALI Department of Plant Breeding and Genetics, Faculty of Agricultural Sciences, University of the Punjab, P.O BOX. 54590, Lahore, Pakistan
M ASHFAQ Department of Plant Breeding and Genetics, Faculty of Agricultural Sciences, University of the Punjab, P.O BOX. 54590, Lahore, Pakistan
A AHMAD Department of Clinical Pharmacy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia
A ULLAH Department of Allied Health Sciences, Iqra National University (INU) Peshawar, 25000 Peshawar, Pakistan

DOI:

https://doi.org/10.64013/bbasr.v2025i1.105

Keywords:

Industrial wastewater, Polyhydroxyalkanoates (PHA), Serratia nematodiphila, Pseudomonas granadensis, Enterobacter cloacae, 16s RNA

Abstract

Due to high organic load and potential toxicity, the industrial wastewater (WW) poses significant environmental challenges necessitating effective management strategies. The WW presents a unique prospect for bioprocessing such as for the production of polyhydroxyalkanoates (PHAs) due to the presence of microorganisms. This study aimed to isolate and characterize PHA-producing microbes from industrial WW, evaluating their potential for biopolymer synthesis. The collected WW samples were analyzed physiochemically to determine values of parameters like temperature, pH, and concentrations of TDS, BOD and chemical oxygen demand (COD). Results of this research work shows the temperature 40^oC, pH =6.2, BOD 3900mg/L, COD 6960m/L and TDS 868mg/L, showing a high number of pollutants adequate for microbial growth. Total of nine strains of bacteria were isolated, among which three strains are recognized as PHA producers that are Serratia nematodiphla, Pseudomonas granadensis and Enterobacter cloacae. These strains were identified through staining techniques and molecular characterization using 16S rRNA sequencing, UV Visible spectroscopy determined characteristics. A total indicated characteristic absorbance peaks corresponding to PHA, while FTIR analysis identified functional groups indicative of biopolymeric structures. The investigations determined that WW is an efficient substrate for growing PHA producing bacteria, providing sustainable waste management and non-toxic materials. In future researcher should focus on various factors to create an ideal environment for cultivation and increasing the capacity for PHA production. This approach turns waste into valuable products and advancing circular economy.

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