ANTIMICROBIAL ACTIVITY OF LANTANA CAMARA AGAINST PSEUDOMONAS AERUGINOSA, SERRATIA MARCESCENS AND STAPHYLOCOCCUS AUREUS TO DEVELOP OINTMENT BASED THERAPY

Authors

  • SZU DIN Institute of Microbiology and Molecular Genetics, University of The Punjab, Lahore, Pakistan
  • MO FAZAL Punjab Medical Collage, Faisalabad Medical University, Faisalabad, Pakistan
  • A ISHTIAQUE Department of Medicine, King Edward Medical University, Lahore Pakistan
  • A ULLAH Department of Health and Biological Sciences, Abasyn University Peshawar, Khyber Pakhtunkhwa, Pakistan

DOI:

https://doi.org/10.54112/bbasr.v2023i1.33

Keywords:

Lantana camara,, Pheretima posthuman, pathogenic bacteria, antibacterial, organic extracts

Abstract

Plant extract (Lantana camara) and earthworm (Pheretima posthuma) extract have been used to treat many illnesses for centuries; they have been reported to suppress the growth of many pathogenic bacteria and are specially used for wound healing. We analyzed the antibacterial activity of the different organic extracts obtained from these species against pathogenic strains of bacteria and compared them to know which is more potent for developing antiseptic ointment. Ethanol and acetone solvents were used for the extraction and analyzed for their antibacterial activity by the disk diffusion and shake flask test. Then we identified the minimum inhibitory concentration of extracts against gram-positive and gram-negative strains. Lantana camara showed the largest growth inhibitory zone against Pseudomonas aeruginosa, followed by Serratia marcescens, and Staphylococcus aureus. MIC for plant extract was 150μg/ml, while earthworm extract inhibited growth at a minimum dose of 200μg/ml. We found that the plant's ethanol extract (L. camara) exhibited robust antimicrobial activity compared to the worm extract. Current investigation reinforces the application of Lantana camara extract to develop ointment to treat wound infections.

References

Bhorgin, A., & Uma, K. (2014). Antimicrobial activity of earthworm powder (Lampito mauritii). Int. J. Curr. Microbiol. App. Sci, 3(1), 437-443.

Byzov, B. A., Khomyakov, N. V., Kharin, S. A., & Kurakov, A. V. (2007). Fate of soil bacteria and fungi in the gut of earthworms. European Journal of Soil Biology, 43, S149-S156. DOI: https://doi.org/10.1016/j.ejsobi.2007.08.012 DOI: https://doi.org/10.1016/j.ejsobi.2007.08.012

Cho, J. H., Park, C. B., Yoon, Y. G., & Kim, S. C. (1998). Lumbricin I, a novel proline-rich antimicrobial peptide from the earthworm: purification, cDNA cloning and molecular characterization. Biochimica et Biophysica Acta (BBA)-Molecular basis of disease, 1408(1), 67-76. DOI: https://doi.org/10.1016/S0925-4439(98)00058-1 DOI: https://doi.org/10.1016/S0925-4439(98)00058-1

Cruz, C., Moreno, J., Renzoni, A., Hidalgo, M., Reyes, J., Schrenzel, J., . . . Arias, C. A. (2005). Tracking methicillin-resistant Staphylococcus aureus clones in Colombian hospitals over 7 years (1996–2003): emergence of a new dominant clone. International journal of antimicrobial agents, 26(6), 457-462. DOI: https://doi.org/10.1016/j.ijantimicag.2005.08.013 DOI: https://doi.org/10.1016/j.ijantimicag.2005.08.013

Deena, M., & Thoppil, J. (2000). Antimicrobial activity of the essential oil of Lantana camara. Fitoterapia, 71(4), 453-455. DOI: https://doi.org/10.1016/S0367-326X(00)00140-4 DOI: https://doi.org/10.1016/S0367-326X(00)00140-4

Kannan, R., Shackleton, C. M., & Shaanker, R. U. (2013). Reconstructing the history of introduction and spread of the invasive species, Lantana, at three spatial scales in India. Biological Invasions, 15(6), 1287-1302. OI: https://doi.org/10.1007/s10530-012-0365-z DOI: https://doi.org/10.1007/s10530-012-0365-z

Nayak, B., Raju, S., & Ramsubhag, A. (2008). Investigation of wound healing activity of Lantana camara in Sprague Dawley Rats using a burn wound model. Int J Appl Res Nat Pro, 1(1), 15-19.

Nirmal, C. R., Ebenezer, R. S., Kannan, P., Balasubramanian, M., Thirunavukkarasu, I., Mondal, R., & Dusthackeer, A. (2020). Anti-tuberculosis activity of bio-active compounds from Lantana camara L., Euphorbia hirta L., Mukia maderaspatana (L.) M. Roem, and Abutilon indicum (L.). European Journal of Integrative Medicine, 35, 101105. DOI: https://doi.org/10.1016/j.eujim.2020.101105 DOI: https://doi.org/10.1016/j.eujim.2020.101105

Rios, J.-L., Recio, M. C., & Villar, A. (1988). Screening methods for natural products with antimicrobial activity: a review of the literature. Journal of ethnopharmacology, 23(2-3), 127-149. DOI: https://doi.org/10.1016/0378-8741(88)90001-3

Yau, Y. H., Ho, B., Tan, N. S., Ng, M. L., & Ding, J. L. (2001). High therapeutic index of factor C Sushi peptides: potent antimicrobials against Pseudomonas aeruginosa. Antimicrobial agents and chemotherapy, 45(10), 2820-2825. DOI: https://doi.org/10.1128/AAC.45.10.2820-2825.2001 DOI: https://doi.org/10.1128/AAC.45.10.2820-2825.2001

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Published

2023-03-09

How to Cite

DIN, S., FAZAL, M., ISHTIAQUE, A., & ULLAH, A. (2023). ANTIMICROBIAL ACTIVITY OF LANTANA CAMARA AGAINST PSEUDOMONAS AERUGINOSA, SERRATIA MARCESCENS AND STAPHYLOCOCCUS AUREUS TO DEVELOP OINTMENT BASED THERAPY. Bulletin of Biological and Allied Sciences Research, 2023(1), 33. https://doi.org/10.54112/bbasr.v2023i1.33

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