CHLOROPHYTUM COMOSUM-MEDIATED IRON NANOPARTICLES: AN ECO-FRIENDLY APPROACH FOR ANTIMICROBIAL AND DYE DEGRADATION APPLICATIONS

REZ KAYANI; A RUSTAM; U SALEEM; M BILAL; M BAKHTIYAR; S JAMSHAID; M SOHAIL; K FAROOQ; S KHAN; B REHMAN

doi:10.54112/bbasr.v2025i1.94

Authors

REZ KAYANI Department of Allied Health Sciences, Molecular Biology Laboratory, Iqra National University (INU) Peshawar, 25000 Peshawar, Pakistan
A RUSTAM Department of Allied Health Sciences, Molecular Biology Laboratory, Iqra National University (INU) Peshawar, 25000 Peshawar, Pakistan
U SALEEM Department of Allied Health Sciences, Molecular Biology Laboratory, Iqra National University (INU) Peshawar, 25000 Peshawar, Pakistan
M BILAL Department of Allied Health Sciences, Molecular Biology Laboratory, Iqra National University (INU) Peshawar, 25000 Peshawar, Pakistan
M BAKHTIYAR Department of Allied Health Sciences, Molecular Biology Laboratory, Iqra National University (INU) Peshawar, 25000 Peshawar, Pakistan
S JAMSHAID Department of Allied Health Sciences, Molecular Biology Laboratory, Iqra National University (INU) Peshawar, 25000 Peshawar, Pakistan
M SOHAIL Department of Health and Biological Sciences, Iqra National University (INU) Peshawar, 25000 Peshawar, Pakistan
K FAROOQ Department of Pharmacy, Capital University of Science and Technology Islamabad, 4550 Islamabad, Pakistan
S KHAN School of Resources and Environmental Engineering, East China University of Science and Technology Shanghai, 200237, China
B REHMAN Department of Health and Biological Sciences, Iqra National University (INU) Peshawar, 25000 Peshawar, Pakistan

DOI:

https://doi.org/10.54112/bbasr.v2025i1.94

Keywords:

iron nanoparticles (FeNPs), Chlorophytum comosum, Methyl Orange (MO) degradation, SEM, ultraviolet-visible (UV-Vis) spectroscopy, EDAX, FTIR

Abstract

Green synthesis techniques are becoming more and more popular in nanotechnology because of their many advantages, which include great efficiency, affordability, and environmental friendliness. Here, iron nanoparticles (FeNPs) were prepared using the methanolic extract of Chlorophytum comosum leaf. The results were spherical and amorphous FeNPs, with a particle size of around 50 nm, as validated by SEM. Energy Dispersive X-ray Analysis EDAX revealed the presence of Iron (Fe) in the sample. The peaks in the FTIR spectrum of aqueous extract of C.comosum at 3367.15cm, 2076.08cm, 2162.58cm, 1996.39cm, 2021.91cm, 475.58cm, 456.57cm,456.58cm, 418.05cm, 430.34cm, 410.21cm were OH, Carbohydrates proteins and polyphenols, Silicon Compound, Alkene conjugated triple bond, Aromatics, Silicon Compounds and Cycloalkanes. Methyl Orange (MO) was successfully removed from the aqueous solution by the synthesized FeNPs. Using ultraviolet-visible (UV-Vis) spectroscopy, it is simple to monitor the concentration of MO while using FeNPs in the presence of H₂O₂. After 6 hours, the synthesized FeNPs showed an 83% MO degradation efficiency. Additionally, several Gram-positive and Gram-negative bacteria as well as fungus species including Aspergillus were used to test the antibacterial and antifungal properties of these FeNPs. The results indicate that FeNPs have a bactericidal effect on both Gram-negative Pseudomonas aeruginosa (the zone of inhibition is 11mm to 16mm) and Gram-positive Staphylococcus epidermidis (the lowest zone of inhibition is 12mm and the greatest is 18mm). Additionally, the range of Penicillium's zone of inhibition is 12 mm to 17 mm, while the lowest and greatest zones of inhibition against Aspergillus Niger are 17 mm and 20 mm, respectively. The substantial bactericidal effect of these INPs on both Gram-positive and Gram-negative bacteria as well as on fungi was demonstrated by the results of their antibacterial and antifungal activity. All things considered, ecologically friendly FeNPs can be a good option for several scientific domains, especially the elimination of organic dyes and the eradication of germs and fungus.

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References

Akbari, A., Sabouri, Z., Hosseini, H. A., Hashemzadeh, A., Khatami, M., &Darroudi, M. (2020). Effect of nickel oxide nanoparticles as a photocatalyst in dyes degradation and evaluation of effective parameters in their removal from aqueous environments. Inorganic Chemistry Communications, 115, 107867. https://doi.org/10.1016/j.inoche.2020.107867 DOI: https://doi.org/10.1016/j.inoche.2020.107867

Cao, Y., Dhahad, H. A., El-Shorbagy, M. A., Alijani, H. Q., Zakeri, M., Heydari, A.,..&Dehkordi, F. F. (2021). Green synthesis of bimetallic ZnO–CuO nanoparticles and their cytotoxicity properties. Scientific Reports, 11(1), 23479. https://doi.org/10.1038/s41598-021-02937-1 DOI: https://doi.org/10.1038/s41598-021-02937-1

Dhuper, S., Panda, D., &Nayak, P. L. (2012). Green synthesis and characterization of zero valent iron nanoparticles from the leaf extract of Mangiferaindica. Nano Trends: J Nanotech App, 13(2), 16-22.

David, A., Luydmila, L., & Maria, K. (2013). The use of enzymatic hydrolyzate of C.comosum with experimental toxic liver damage in rats. Am J Biomed Life Sci, 1,32-6. https://doi.org/10.11648/j.ajbls.20130101.16 DOI: https://doi.org/10.11648/j.ajbls.20130101.16

Gawrońska, H., &Bakera, B. (2015). Phytoremediation of particulate matter from indoor air by C.comosum L. plants. Air Quality, Atmosphere & Health, 8(3), 265-272. https://doi.org/10.1007/s11869-014-0285-4 DOI: https://doi.org/10.1007/s11869-014-0285-4

G. Vilardi, M. Parisi, N. Verdone, Simultaneous aggregation and oxidation of nZVI in Rushton equipped agitated vessel: experimental and modelling, Powder Technol 353 (2019) 238. https://doi.org/10.1016/j.powtec.2019.05.033 DOI: https://doi.org/10.1016/j.powtec.2019.05.033

Gobalakrishnan, S., Chidhambaram, N., &Chavali, M. (2021). Role of greener syntheses at the nanoscale. In Handbook of greener synthesis of nanomaterials and compounds (pp. 107-134). Elsevier. https://doi.org/10.1016/b978-0-12-821938-6.00004-9 DOI: https://doi.org/10.1016/B978-0-12-821938-6.00004-9

Huber, D. L. (2005). Synthesis, properties, and applications of ironnanoparticles. Small, 1(5), 482-501. https://doi.org/10.1002/smll.200500006 DOI: https://doi.org/10.1002/smll.200500006

L. Wang, C. Hu, L. Shao, The antimicrobial activity of nanoparticles: present situation and prospects for the future, Int. J. Nanomed. 12 (2017) 1227. https://doi.org/10.2147/ijn.s121956 DOI: https://doi.org/10.2147/IJN.S121956

Lee, S. H., & Jun, B. H. (2019). Silver nanoparticles: synthesis and application for nanomedicine. International journal of molecular sciences, 20(4), 865. https://doi.org/10.3390/ijms20040865 DOI: https://doi.org/10.3390/ijms20040865

Njagi, E. C., Huang, H., Stafford, L., Genuino, H., Galindo, H. M., Collins, J. B., ... &Suib, S. L. (2011). Biosynthesis of iron and silver nanoparticles at room temperature using aqueous sorghum bran extracts. Langmuir, 27(1), 264-271. https://doi.org/10.1021/la103190n DOI: https://doi.org/10.1021/la103190n

Nahari, M. H., Al Ali, A., Asiri, A., Mahnashi, M. H., Shaikh, I. A., Shettar, A. K., &Hoskeri, J. (2022). Green Synthesis and Characterization of Iron Nanoparticles Synthesized from Aqueous Leaf Extract of Vitexleucoxylon and Its Biomedical. Applications. Nanomaterials, 12(14), 2404.. https://doi.org/10.3390/nano12142404 DOI: https://doi.org/10.3390/nano12142404

Rzhepakovsky, I. V., Areshidze, D. A., Avanesyan, S. S., Grimm, W. D., Filatova, N. V., Kalinin, A. V., ... &Piskov, S. I. (2022). Phytochemical characterization,antioxidant activity, and cytotoxicity of methanolic leaf extract of C.Comosum (green type)(Thunb.) Jacq. Molecules, 27(3), 762. https://doi.org/10.3390/molecules27030762 DOI: https://doi.org/10.3390/molecules27030762

Shahwan, T., Sirriah, S. A., Nairat, M., Boyacı, E., Eroğlu, A. E., Scott, T. B., & Hallam, K. R. (2011). Green synthesis of iron nanoparticles and their application as a Fenton-like catalyst for the degradation of aqueous cationic and anionic dyes. Chemical Engineering Journal, 172(1), 258-266. https://doi.org/10.1016/j.cej.2011.05.103 DOI: https://doi.org/10.1016/j.cej.2011.05.103

Shah, S., Dasgupta, S., Chakraborty, M., Vadakkekara, R., &Hajoori, M. (2014). Green synthesis of iron nanoparticles using plant extracts. Int J Biol Pharm Res, 5(7), 549-52

Shah, S. T., Chowdhury, Z. Z., Simarani, K., Basirun, W. J., Badruddin, I. A., Hussien, M., ... & Kamangar, S. (2022). Nanoantioxidants: The Fourth Generation of Antioxidants—Recent Research Roadmap and Future Perspectives. Coatings, 12(10), 1568. https://doi.org/10.3390/coatings12101568 DOI: https://doi.org/10.3390/coatings12101568

Treesubsuntorn, C., Lakaew, K., Autarmat, S., &Thiravetyan, P. (2020). Enhancing benzene removal by C.comosum under simulation microgravity system: Effect of light-dark conditions and indole-3-acetic acid. ActaAstronautica, 175,396-404. https://doi.org/10.1016/j.actaastro.2020.05.061 DOI: https://doi.org/10.1016/j.actaastro.2020.05.061