IN VITRO CONTROL OF POST-HARVEST FRUITS ROT PATHOGENIC FUNGI USING AZADIRACHTA INDICA (NEEM) SEEDS AND LEAVES EXTRACTS

J ALI; A HUSSAIN; J ABBAS

doi:10.54112/bbasr.v2025i1.102

Authors

J ALI Food Technology Center, Pakistan Council of Scientific and Industrial Research (PCSIR) Laboratories Complex, Peshawar 25120- Pakistan
A HUSSAIN Food Technology Center, Pakistan Council of Scientific and Industrial Research (PCSIR) Laboratories Complex, Peshawar 25120- Pakistan
J ABBAS Food Technology Center, Pakistan Council of Scientific and Industrial Research (PCSIR) Laboratories Complex, Peshawar 25120- Pakistan

DOI:

https://doi.org/10.54112/bbasr.v2025i1.102

Keywords:

Plant pathogenic fungi, Post-harvest fungal diseases, spore inhibition, colony diameter, mycelial growth inhibition

Abstract

The present study was carried out to assess the neem seeds and leaves ethanol and aqueous extracts against post-harvest fruits rot pathogenic fungi i.e. Alternarta citrt, Dothiorella dominicana, Lasiodiplodia theobromae, Colletotrichum loeosporioides, Phomopsis caricae-papayae and Colletotrichum musae. The mycelial growth inhibition assay, spore germination, and spore inhibition germination assay in groove slides were determined. The results showed that the neem seed ethanol extracts showed maximum mycelium growth reduction of 88.16% against Colletotrichum musae, while the minimum mycelium growth inhibition was 51.32% against Lasiodiplodia theobromae. The neem leaf extracts maximum and minimum spore inhibition was calculated 66% (ethanol) and 40% (aqueous) against Colletotrichum musae and Lasiodiplodia theobromae respectively. The neem leaf ethanol extracts showed maximum (70%) spores’ inhibition germination against Colletotrichum musae and minimum (38%) spores’ inhibition germination was observed against Lasiodiplodia theobromae. It was noted that neem seeds and ethanol extracts were the most effective antifungal parts and solvents as compared with leaves and aqueous extract. The suggested that the neem extract many be used for the control of pathogenic fungi species.

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